Product Description
working principle
A biological fermentation tank uses an air nozzle to spray high-speed air, which is dispersed in a bubble like manner in the liquid. On the ventilated side, the average density of the liquid decreases, while on the non ventilated side, the liquid density is higher, resulting in a density difference with the liquid on the ventilated side, thus forming a circulation of the liquid inside the fermentation tank.
Type characteristics
There are various types of biological fermentation tanks, such as mechanical agitation fermentation tanks, bubble fermentation tanks, liquid circulation fermentation tanks, etc.
1. Mechanical stirring fermentation tanks can be divided into ventilated fermentation tanks, self-priming fermentation tanks, etc. Mechanical stirring and ventilation fermentation tank is a commonly used type in fermentation equipment. Mechanical stirring and ventilation fermentation tank is a sealed pressure equipment, whose main components include tank body, shaft seal, defoamer, agitator, coupling, intermediate bearing, baffle, air supply pipe, heat exchanger, wellbore, pipeline, etc. (1) Ventilation fermentation tank: Ventilation fermentation tank is commonly used in industry as a microbial reactor. Its advantage is that the pH value and temperature of the fermentation broth are easy to control, making it suitable for continuous cultivation. The disadvantage is that cells are easily damaged during the cultivation of filamentous bacteria.
(2) The self-priming fermentation machine does not require an air compressor to supply compressed air. The self-priming fermentation machine uses negative pressure generated by a stirrer to suck in air. Its advantage is that both stirring and air supply are completed by a mechanical stirrer. The disadvantage is that the suction range is not high, and fermentation with high sterile requirements is not suitable because air enters the fermenter during stirring, which is not suitable for anaerobic bacteria fermentation
2. Bubble fermentation tank
Utilize bubbling air to provide the necessary power for mixing and mass transfer. Bubble fermentation tanks have the advantages of simple structure, low cost, and low power consumption, which reduce the harm of filamentous bacterial culture. Bubble fermentation tanks are generally suitable for fermentation processes with low viscosity, low solid content, and low oxygen consumption
3. Liquid circulation awakening tank
There are two types of liquid circulation fermentation tanks: gas lift fermentation tanks and nozzle ring fermentation tanks
(1) Airlift fermentation tank: Airlift fermentation tank is similar to bubble fermentation, without mechanical stirring, but with an external circulation pipe outside the tank. Gas lift fermentation tanks have the advantages of simple structure, low cost, and low energy consumption. Gas lift fermentation tanks are generally suitable for low viscosity and low solid content culture media
(2) Nozzle Loop Fermentation Tank: The nozzle loop fermentation tank uses a mechanical pump to discharge compressed air, forming a shear force field at the nozzle to disperse the injected air into small bubbles in the liquid phase. Compared to gas lift fermentation tanks, nozzle ring fermentation tanks are more suitable for a wider range.
usage method
1. Correct pH electrode and dissolved oxygen electrode.
2. Sterilization of tanks. Add the culture medium into the tank as needed, seal it as required, and place the small fermentation tank (5L) in a large sterilization pot for sterilization (115C, 30 minutes); Large fermentation tanks (10L and above) are sterilized with steam (121C, 30min)
3. After the tank has cooled down, place it on the fermentation platform, install it properly, turn on the cooling water, turn on the power of the air pump, connect the ventilation pipe to start ventilation, adjust the air intake knob to adjust the ventilation volume appropriately, and keep the tank at 0.05MPa. Set the temperature, H, Mixing speed, etc., and calibrating the slope value of the dissolution electrode to 100% at a determined speed and ventilation rate
4. After the temperature stabilizes and all parameters are correct, connect the pre shaken seeds, start fermentation timing, and record various parameters. After fermentation is complete, clean the tank and electrodes, insert the pH electrode into a triangular flask containing 3M potassium chloride for later use, and protect the probe of the dissolved oxygen electrode with a protective sleeve for storage.
Product applications: food, dairy, pharmaceutical, chemical, biological, oil-water, etc.
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